Honeybee populations have been in serious decline for years, and Purdue University scientists may have identified one of the factors that cause bee deaths around agricultural fields. Analyses of bees found dead in and around hives from several apiaries（蜂房） over two years in Indiana showed the presence of neonicotinoid insecticides, which are commonly used to coat corn and soybean seeds before planting. The research showed that those insecticides were present at high concentrations in waste talc（滑石，云母） that is exhausted from farm machinery during planting.

The insecticides clothianidin and thiamethoxam were also consistently found at low levels in soil -- up to two years after treated seed was planted -- on nearby dandelion（蒲公英） flowers and in corn pollen（花粉） gathered by the bees, according to the findings released in the journal PLoS One this month.

"We know that these insecticides are highly toxic to bees; we found them in each sample of dead and dying bees," said Christian Krupke, associate professor of entomology and a co-author of the findings.

The United States is losing about one-third of its honeybee hives each year, according to Greg Hunt, a Purdue professor of behavioral genetics, honeybee specialist and co-author of the findings. Hunt said no one factor is to blame, though scientists believe that others such as mites and insecticides are all working against the bees, which are important for pollinating food crops and wild plants.

"It's like death by a thousand cuts for these bees," Hunt said.

Krupke and Hunt received reports that bee deaths in 2010 and 2011 were occurring at planting time in hives near agricultural fields. Toxicological screenings performed by Brian Eitzer, a co-author of the study from the Connecticut Agricultural Experiment Station, for an array of pesticides showed that the neonicotinoids used to treat corn and soybean seed were present in each sample of affected bees. Krupke said other bees at those hives exhibited tremors, uncoordinated movement and convulsions（抽搐） , all signs of insecticide poisoning.

Seeds of most annual crops are coated in neonicotinoid insecticides for protection after planting. All corn seed and about half of all soybean seed is treated. The coatings are sticky, and in order to keep seeds flowing freely in the vacuum systems used in planters, they are mixed with talc. Excess talc used in the process is released during planting and routine planter cleaning procedures.