The Phalaenopsis orchid, also known as the moth orchid, is the most important pot flower（盆花） in terms of market value in the world's major floriculture（花卉栽培） markets. Because nitrogen significantly affects the growth and flowering of Phalaenopsis, nitrogen needs during flowering are of particular interest to growers. Researchers Hadi Susilo, Ying-Chun Peng, and Yao-Chien Alex Chang from the Department of Horticulture and Landscape Architecture at National Taiwan University published a study in the Journal of the American Society for Horticultural Science that determined the importance of providing Phalaenopsis with ample（丰富的） nitrogen fertilization at the its various growth stages. "To our knowledge, the relative contributions of stored nitrogen (N) and recently absorbed fertilizer N to the developing inflorescence had not been studied in Phalaenopsis," the authors said. "The relative contributions to the stored N pool of N accumulated during the different growth stages during the long vegetative period of Phalaenopsis cultivation were also unknown. The objective of our study was to bring answers to these unknowns."

The team used N-labeling--a powerful research tool for accurately determining the fate of nitrogen in the environment--to compare the contributions of fertilizer N, applied before or after spiking, to the developing inflorescence, and compared the relative contributions of fertilizer N absorbed during various stages of the vegetative period to the stored N pool. "The nutritional study of Phalaenopsis is difficult with traditional methods because it has a strong buffering capacity against nutrient deficiency, but we used 15N-labeling with the enrichment method to accurately trace the absorption and partitioning of fertilizer N in Phalaenopsis," the scientists said.

The results of multiple experiments showed that inflorescence is a major N sink during the reproductive stage of Phalaenopsis. "Fertilizer applied during the reproductive stage is a significant N source for the inflorescence development of Phalaenopsis, whereby current fertilizer application supplies 57% of the N required for inflorescence development. Therefore, providing sufficient fertilizer N is important during the reproductive stage to ensure the quality of flowering," the authors said.

Further experiments showed that, even when ample fertilization was provided during the reproductive stage, 6-8% of the nitrogen accumulated during growing Stage I (small plant in 4.5-cm pot for 5 months), Stage II (medium plant in 8.5-cm pot for 5 months), and Stage III (large plant in 10.5-cm pot for 5 months) ended up in the inflorescence at the visible bud stage. At the "two-third flowers open" stage, 12-16% of the N accumulated in the plant during these three stages ended up in the inflorescence. The scientists concluded that these results indicated the importance of providing Phalaenopsis with ample N fertilization at all growth stages.