Flowers aren't just pretty to look at, they are how plants reproduce. In agricultural plants, the timing and regulation of flower formation has economic significance, affecting a crop's yield. A new paper by researchers at the University of Pennsylvania published in the journal Science has revealed that a plant hormone once believed to promote flower formation in annual plants also plays a role in inhibiting flowers from forming. The dual role of this hormone, gibberellin（赤霉素）, could be exploited to produce higher-yielding crop plants.

The study was led by Nobutoshi Yamaguchi and Doris Wagner of the School of Arts and Sciences' Department of Biology. Wagner is professor and graduate chair, and Yamaguchi is a postdoctoral researcher. Department co-authors included Cara M. Winter, Miin-Feng Wu and Ayako Yamaguchi. The Penn team collaborated with Yuri Kanno and Mitsunori Seo of RIKEN Center for Sustainable Resource Science in Japan.

Plant scientists used to think that short-lived plants, annuals or bi-annuals, use a different strategy from long-lived plants, perennials（多年生植物）, to regulate flower production.

"Anecdotal evidence was that the hormone gibberellin promoted the switch to flower formation in short-lived plants, along with other cues such as temperature, season and photoperiod," Wagner said. "But in the long-lived plants, like in fruit trees, people have known that if you sprayed them with the hormone it inhibited flower production. So it was a big puzzle: why would the same hormone do one thing in short-lived plants and another in long-lived plants?"

To address this paradox, the Penn team began by looking for new genes important to the flower-forming process. Specifically, they performed a genome-wide search of the plant species Arabidopsis thaliana to find direct targets of the protein LEAFY, which is known to promote flower formation.

One gene that turned up was called ELA1, which produces a cytochrome enzyme and has been shown to play a role in breaking down gibberellin. Further experiments showed that in plants that lost ELA1 function, flowers formed much later than normal.

The researchers also found that plants that lacked LEAFY had high levels of gibberellin, and plants engineered to produce high levels of LEAFY had lower levels of the hormone and were also shorter with greater levels of chlorophyll -- characteristics of gibberellin deficiency.

"At first we were confused because gibberellin was supposed to promote all of this activity that leads to flower formation," Wagner said. "Then when we found a direct target of LEAFY that is linked to gibberellin catabolism（分解代谢）, that gave us the clue that gibberellin must have a role in inhibiting flower formation as well."